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mir sensor assay  (Addgene inc)


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    Addgene inc mir sensor assay
    Mir Sensor Assay, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mir+sensor+assay/pmirGLO-Bmpr2-1719+(Plasmid+%2349379)/bio_rxiv__2025__11__21__689335-78-11-18
    Average 93 stars, based on 1 article reviews
    mir sensor assay - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: MIR17HG Expression Is Transcriptionally Regulated by PAX3::FOXO1 and MYCN and is Necessary for Oncogenic Activity in Fusion-Positive Rhabdomyosarcoma
    Article Snippet: .. To evaluate the efficacy of the miRNA-sponges , we used the miR-sensor assay ( ) in pmirGLO vector (Addgene plasmid #49379). ..



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    Representative images of DAPI (blue), F-Actin (magenta), and YAP (white) stained MSCs post transfection with miRNA mimics for <t>miR29a</t> or a scrambled mimic (A). Nontransfected MSC group used as control and the nuclear to cytoplasmic ratio of YAP and the cell area was quantified (B, C). (Violin plots indicate mean, quartiles and distribution, n = 132–163 cells/grp). One-way ANOVA with Dunnett’s post hoc ** p < 0.01, ** p < 0.001. Scale for all images = 50 μm.
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    Image Search Results


    Representative images of DAPI (blue), F-Actin (magenta), and YAP (white) stained MSCs post transfection with miRNA mimics for miR29a or a scrambled mimic (A). Nontransfected MSC group used as control and the nuclear to cytoplasmic ratio of YAP and the cell area was quantified (B, C). (Violin plots indicate mean, quartiles and distribution, n = 132–163 cells/grp). One-way ANOVA with Dunnett’s post hoc ** p < 0.01, ** p < 0.001. Scale for all images = 50 μm.

    Journal: ACS Omega

    Article Title: miR29a-Loaded Extracellular Vesicles Derived from Human Mesenchymal Stem Cells Inhibit Fibrotic and Inflammatory Signaling

    doi: 10.1021/acsomega.5c03490

    Figure Lengend Snippet: Representative images of DAPI (blue), F-Actin (magenta), and YAP (white) stained MSCs post transfection with miRNA mimics for miR29a or a scrambled mimic (A). Nontransfected MSC group used as control and the nuclear to cytoplasmic ratio of YAP and the cell area was quantified (B, C). (Violin plots indicate mean, quartiles and distribution, n = 132–163 cells/grp). One-way ANOVA with Dunnett’s post hoc ** p < 0.01, ** p < 0.001. Scale for all images = 50 μm.

    Article Snippet: A previously described miR29a miRNA sensor (LSB-hsa-miR-29a-3p, Addgene 103391) was transiently transfected into 3T3 fibroblasts and used for miRNA sensor experiments to test delivery and targeting.

    Techniques: Staining, Transfection, Control

    Diagram showing the miRNA sensor construct. With the addition of miR29 and targeting of the miRNA response element (MRE), expression of mKate is reduced (A). Representative images of LSB-has-miR-29a-3p sensor-transfected NIH-3T3 fibroblasts stained with 650 Nuclear Dye after 24 h treatment with Neg. MSC EVs or miR29a mimic-transfected MSC EVs (B). The mKate to BFP signal intensity ratio was quantified for cells treated with MSC EVs (C). (mean ± SEM, n = 106–123 cells/grp). One-way ANOVA with Dunnett’s post hoc * p < 0.05. Scale for all images = 50 μm.

    Journal: ACS Omega

    Article Title: miR29a-Loaded Extracellular Vesicles Derived from Human Mesenchymal Stem Cells Inhibit Fibrotic and Inflammatory Signaling

    doi: 10.1021/acsomega.5c03490

    Figure Lengend Snippet: Diagram showing the miRNA sensor construct. With the addition of miR29 and targeting of the miRNA response element (MRE), expression of mKate is reduced (A). Representative images of LSB-has-miR-29a-3p sensor-transfected NIH-3T3 fibroblasts stained with 650 Nuclear Dye after 24 h treatment with Neg. MSC EVs or miR29a mimic-transfected MSC EVs (B). The mKate to BFP signal intensity ratio was quantified for cells treated with MSC EVs (C). (mean ± SEM, n = 106–123 cells/grp). One-way ANOVA with Dunnett’s post hoc * p < 0.05. Scale for all images = 50 μm.

    Article Snippet: A previously described miR29a miRNA sensor (LSB-hsa-miR-29a-3p, Addgene 103391) was transiently transfected into 3T3 fibroblasts and used for miRNA sensor experiments to test delivery and targeting.

    Techniques: Construct, Expressing, Transfection, Staining

    HDF-seeded collagen gels after 1 week of contraction with treatment of Scr. or miR29a EVs at 1e8 EV/gel (A). (scale bar = 5 mm). Quantification of area of gel normalized to the area of well and the no EV group (B). (mean ± SEM, n = 7 gels/grp). Fold change in CTGF expression of collagen gels (C). (mean ± SEM, n = 3/grp). Representative images of HDF-seeded collagen gels stained with Hoechst (blue), F-Actin (green), and YAP (white) (D). (scale bar = 50 μm). YAP quantification of cells seeded within collagen gels after contraction (E). (mean ± SEM, One-way ANOVA with Dunnett’s post hoc, * p <0.05, *** p <0.001).

    Journal: ACS Omega

    Article Title: miR29a-Loaded Extracellular Vesicles Derived from Human Mesenchymal Stem Cells Inhibit Fibrotic and Inflammatory Signaling

    doi: 10.1021/acsomega.5c03490

    Figure Lengend Snippet: HDF-seeded collagen gels after 1 week of contraction with treatment of Scr. or miR29a EVs at 1e8 EV/gel (A). (scale bar = 5 mm). Quantification of area of gel normalized to the area of well and the no EV group (B). (mean ± SEM, n = 7 gels/grp). Fold change in CTGF expression of collagen gels (C). (mean ± SEM, n = 3/grp). Representative images of HDF-seeded collagen gels stained with Hoechst (blue), F-Actin (green), and YAP (white) (D). (scale bar = 50 μm). YAP quantification of cells seeded within collagen gels after contraction (E). (mean ± SEM, One-way ANOVA with Dunnett’s post hoc, * p <0.05, *** p <0.001).

    Article Snippet: A previously described miR29a miRNA sensor (LSB-hsa-miR-29a-3p, Addgene 103391) was transiently transfected into 3T3 fibroblasts and used for miRNA sensor experiments to test delivery and targeting.

    Techniques: Expressing, Staining